1. Before sampling, confer with the laboratory performing the analysis and review the chain-of-custody requirements as outlined in this SOP to ensure that they understand the procedures and requirements.

2. Obtain, preserve, and handle ground water samples as per NDEE SOPs, EPA method and laboratory requirements.

3. Immediately before or after obtaining an individual sample set, begin filling out the chain-of-custody record.

4. For specific events (e.g. point source incidents or contaminant site investigations), fill out one COC record for each site, completing one line of the upper block on the first page for each specific sample set taken. For larger sites with a large number of samples, fill out a separate COC for each the samples in each sample cooler. For larger, regional sampling events (e.g. GWMA studies), fill out a separate record(s) for each day of sampling.

5. Fill out the sections regarding the sample ID, the date and time the sample was taken, the media/ type of sample, the requested sample parameters and methods and any preservation techniques used. Remarks and notes are optional. For longer-term (e.g. GWMA) studies or sampling events for which these sections are known in advance, the forms may be pre-printed with the appropriate areas filled in.

6. Upon completion of the sampling event, transport the samples to the laboratory for analysis. If the samples are delivered to the lab by the same individual(s) who performed the sampling, the sampler is responsible for maintaining the record until the samples are delivered to the lab. If transferring the samples to a different individual for delivery, the sampler must sign on the lower block on the second page under “Relinquished By.” The person transporting the samples to the lab must sign on the lower block under “Received By,” and fill in the correct date and time in the space provided. If the samples are shipped via commercial carrier to the lab, the sampler must sign in the lower block on the second page under “Delivered By,” and fill in the date and time in the space provided. The COC is then placed in a Ziploc type bag in the top of the cooler. The sample cooler is addressed and sealed. The sampler retains the carrier’s shipping receipt as evidence. The commercial carrier’s delivery tracking may, in this case, be used to further confirm the integrity of the COC and delivery.

7. Upon arrival at the lab, deliver the samples to the individual responsible for sample receipt. Confirm with this individual the analytical requirements for your samples, if you have not already done so.

8. Sign the chain-of-custody record on the lower block under relinquished by.

9. Ensure that the laboratory representative signs the record on the lower block on the second page under “Received By,” and places the correct date and time in the spaces provided. Also, instruct the laboratory representative to fill in the laboratory numbers of the samples at an appropriate time in the space provided (NOTE: if samples are to be delivered by commercial carrier, be sure to instruct the laboratory representative to follow Steps 4-6 in your absence).

10. If delivering the samples to the lab. Follow the labs sample check in process as needed.

11. If the samples must be transferred for any reason (e.g. to another laboratory or section for specialized analyses, etc.), instruct the laboratory representative to follow the above procedure to fill out additional lines on the lower block.

12. Ensure that the lab returns the properly completed chain-of-custody record and file it with the laboratory results until it is no longer needed.

It is possible, but not ideal to have more than two persons receiving and relinquishing the samples. An additional form may need to be added if more than three persons receive and relinquish the samples. Generally, the fewer times the samples change hands, the better it is considered for sample integrity.